|Specificity:||The antibody 9E10 may be used to detect the c-Myc tag.
The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
|Immunogen:||Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.|
|Species Reactivity:||Human, Recognizes fusion proteins in all species|
Recommended dilution: 1-5 μg/ml
Application note: Membrane permeabilization is required.
Recommended dilution: 1-5 μg/ml
Application note: not suitable for immunoprecipitation of native c-Myc protein
Recommended dilution: 0,5-2 μg/ml
Positive control: c-Myc tagged protein
Immunohistochemistry (paraffin sections)
Recommended dilution: 5-10 μg/ml
Positive tissue: perfused brain sections, liver, spleen
|Purity:||> 95% (by SDS-PAGE)|
|Purification:||Purified by protein A|
|Storage Buffer:||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Storage / Stability:||Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.|
|Expiration:||See vial label|
|Lot Number:||See vial label|
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For laboratory research only, not for drug, diagnostic or other use.
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