| Clone: | Hs-3 |
|---|---|
| Isotype: | Mouse IgG1 |
| Immunogen: | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Species Reactivity: | Human |
| Negative Species: | Porcine, Bovine, Canine (Dog), Feline (Cat) |
| Application: |
Western Blotting
Recommended dilution: 1 μg/ml
Positive material: seminal plasma
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: Sertoli cells
Immunocytochemistry
ELISA
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from ascites by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic. |
| References: |
*Pajak B, Orzechowski A.: Clusterin: the missing link in the calcium-dependent resistance of cancer cells
to apoptogenic stimuli. Postepy Hig Med Dosw (Online). 2006;60:45-51.
*Rodríguez-Piñeiro AM, de la Cadena MP, López-Saco A, Rodríguez-Berrocal F.J.: Differential expression of serum clusterin isoforms in colorectal cancer. Mol Cell Proteomics. 2006 Sep;5(9):1647-57.
*Shannan B, Seifert M, Boothman DA, Tilgen W, Reichrath J.: Clusterin and DNA repair: a new function in cancer for a key player in apoptosis and cell cycle control.
J Mol Histol. 2006 Sep;37(5-7):183-8.
*Cochrane DR, Wang Z, Muramaki M, Gleave ME, Nelson C.C.: Differential regulation of clusterin and its isoforms by androgens in prostate cells. J Biol Chem. 2007 Jan 26;282(4):2278-87.
*Schepeler T, Mansilla F, Christensen LL, Orntoft TF, Andersen C.L.: Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling. J Mol Signal. 2007 Jul 16;2:6.
*Capkova J, Geussova G, Peknicova J.: New monoclonal antibody to human apolipoprotein J. Folia Biol (Praha). 2002;48(1):40-2.
|
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