|Specificity:||The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
|Immunogen:||Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.|
Immunohistochemistry (paraffin sections)
Recommended dilution:10 μg/ml
Positive tissue: spleen
|Purity:||> 95% (by SDS-PAGE)|
|Purification:||Purified from hybridoma culture supernatant by protein A-affinity chromatography.|
|Storage Buffer:||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Storage / Stability:||Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.|
|Expiration:||See vial label|
|Lot Number:||See vial label|
*Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris, July 2003).
*Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pistillo MP: HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol. 2005 Jan;66(1):1-12.
For laboratory research only, not for drug, diagnostic or other use.
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