| Clone: | MEM-G/11 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody MEM-G/11 recognizes HLA-G1 antigen. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. |
| Immunogen: | Recombinant human HLA-G refolded with beta2-microglobulin and peptide. |
| Species Reactivity: | Human |
| Application: |
Immunoprecipitation
Flow Cytometry
The antibody MEM-G/11 does not cross-block standard MEM-G/9 antibody.
Recommended dilution:2 μg/ml
Immunohistochemistry (frozen sections)
ELISA
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| References: |
*Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26.
*Boyson JE, Erskine R, Whitman MC, Chiu M, Lau JM, Koopman LA, Valter MM, Angelisova P, Horejsi V, Strominger JL.: Disulfide bond-mediated dimerization of HLA-G on the cell surface. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16180-5.
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