| Clone: | B31.15 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody B31.15 reacts with human beta Endorphin, an endogenous opiate derived from ACTH gene. ACTH (Corticotropin; human 39 aa) is synthesized by the anterior pituitary gland and stimulates the adrenal cortex; 6 hormones are derived from one ACTH gene: ACTH, lipotropin, alpha-MSH, beta-MSH, endorphin, and one other. |
| Immunogen: | Human beta Endorphin (full length native protein). |
| Species Reactivity: | Human, Other not tested |
| Application: |
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 - 20 μg/ml
Staining technique: Standard ABC technique (DAB+) Pretreatment: Heat retrieval (microwave oven) Incubation: overnight at 4°C Positive tissue: Human pituitary gland
Immunohistochemistry (frozen sections)
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| References: |
*Andjelkov N, Elvenes J, Figenschau Y, Bjorkoy G, Knutsen G, Seternes T, Johansen O: Detection of mRNA transcripts of truncated opiate precursor (POMC) in human cartilage. Cell Biochem Funct. 2006 May-Jun;24(3):229-35.
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