| Clone: | MEM-G/9 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003). |
| Immunogen: | Recombinant human HLA-G refolded with beta2-microglobulin and peptide. |
| Species Reactivity: | Human |
| Negative Species: | Mouse |
| Application: |
Flow Cytometry
Recommended dilution: 1-5 μg/ml
Positive control: JEG-3 human choriocarcinoma cell line
Immunoprecipitation
Immunohistochemistry (frozen sections)
Immunocytochemistry
Recommended dilution:
Alexa Fluor® 488 Fab-fragment: 5 μg/ml
ELISA
Application note: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32.
Coating antibody (10 μg/ml) Detection antibody (biotin or peroxidase conjugate; 1 μg/ml) |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from ascites by precipitation methods. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| References: |
*Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P.: Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens. 2000 Jun;55(6):510-8.
*Lozano JM, Gonzalez R, Kindelan JM, Rouas-Freiss N, Caballos R, Dausset J, Carosella ED, Pena J.: Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule. AIDS. 2002 Feb 15;16(3):347-51.
*Pangault C, Le Friec G, Caulet-Maugendre S, Lena H, Amiot L, Guilloux V, Onno M, Fauchet R.: Lung macrophages and dendritic cells express HLA-G molecules in pulmonary
diseases. Hum Immunol. 2002 Feb;63(2):83-90.
*Fuzzi B, Rizzo R, Criscuoli L, Noci I, Melchiorri L, Scarselli B, Bencini E, Menicucci A, Baricordi OR.: HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur J Immunol. 2002 Feb;32(2):311-5.
*Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N.: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26.
*Abstracts from the 3rd International Conference on HLA-G. Tissue Antigens 62, 339-357 (2003).
*Lopez AS, Alegre E, LeMaoult J, Carosella E, Gonzalez A.
Regulatory role of tryptophan degradation pathway in HLA-G expression by human
monocyte-derived dendritic cells.
Mol Immunol. 2006 Jul;43(14):2151-60.
*Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175(8):4866-74.
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