| Clone: | MEM-108 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody MEM-108 reacts with CD98, a 125 kDa disulfide-linked heterodimer (80 kDa glycosylated heavy chain + 45 kDa non-glykosylated light chain). CD98 is expressed on T lymphocytes (upon activation) and activated NK cells; it is also present at low levels on B lymphocytes, NK cells, monocytes and platelets. HLDA VI; WS Code BP 409 HLDA VI; WS Code NL N-L017 |
| Immunogen: | RAJI human Burkitt's lymphoma cell line |
| Species Reactivity: | Human |
| Preparation: | The purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Storage Buffer: | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
| Storage / Stability: | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user. |
| Usage: | The reagent is designed for Flow Cytometry analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.1 ml) is sufficient for 25 tests. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | CD98 (4F2) is a type II transmembrane glycoprotein which serves as the heavy chain of the heterodimeric amino acid transporters (HATs). CD98, linked to various light chains by disulfide bond, is responsible for cell surface expression and basolateral localization of this transporter complex in polarized epithelial cells and also interacts with beta1 integrins and increases their affinity for ligand. Besides its roles in amino acid transport, CD98 is thus involved in cell fusion and activation. It is implicated in regulation of cellular differentiation, growth and apoptosis. |
| References: |
*Liu X, Charrier L, Gewirtz A, Sitaraman S, Merlin D: CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT-2 in polarized intestinal epithelia. J Biol Chem. 2003 Jun 27;278(26):23672-7.
*Cho JY, Skubitz KM, Katz DR, Chain BM: CD98-dependent homotypic aggregation is associated with translocation of protein kinase Cdelta and activation of mitogen-activated protein kinases. Exp Cell Res. 2003 May 15;286(1):1-11.
*Cai S, Bulus N, Fonseca-Siesser PM, Chen D, Hanks SK, Pozzi A, Zent R: CD98 modulates integrin beta1 function in polarized epithelial cells. J Cell Sci. 2005 Mar 1;118(Pt 5):889-99.
*Dalton P, Christian HC, Redman CW, Sargent IL, Boyd CA: Differential effect of cross-linking the CD98 heavy chain on fusion and amino acid transport in the human placental trophoblast (BeWo) cell line. Biochim Biophys Acta. 2007 Mar;1768(3):401-10.
*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
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