| Clone: | MEM-63 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts. HLDA VI; WS Code AS A047 |
| Immunogen: | NALM-6 human pre-B cell line |
| Species Reactivity: | Human, Porcine |
| Application: |
Flow Cytometry
Recommended dilution:2 μg/ml
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B. |
| References: |
*Bayas MV, Schulten K, Leckband D: Forced detachment of the CD2-CD58 complex. Biophys J. 2003 Apr;84(4):2223-33.
*Veltroni M, De Zen L, Sanzari MC, Maglia O, Dworzak MN, Ratei R, Biondi A, Basso G, Gaipa G; I-BFM-ALL-FCM-MRD-Study Group: Expression of CD58 in normal, regenerating and leukemic bone marrow B cells:
implications for the detection of minimal residual disease in acute lymphocytic leukemia. Haematologica. 2003 Nov;88(11):1245-52.
*Ariel O, Kukulansky T, Raz N, Hollander N: Distinct membrane localization and kinase association of the two isoforms of CD58. Cell Signal. 2004 Jun;16(6):667-73.
*Sheng L, Li J, Qi BT, Ji YQ, Meng ZJ, Xie M: Investigation on correlation between expression of CD58 molecule and severity of hepatitis B. World J Gastroenterol. 2006 Jul 14;12(26):4237-40.
*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
|
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