| Clone: | MEM-111 |
|---|---|
| Isotype: | Mouse IgG2a |
| Specificity: | The antibody MEM-111 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. HLDA VI; WS Code AS A049 |
| Immunogen: | Raji human Burkitt's lymphoma cell line |
| Species Reactivity: | Human, Rat, Bovine |
| Preparation: | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Storage Buffer: | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
| Storage / Stability: | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user. |
| Usage: | The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.5 ml) is sufficient for 25 tests. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. |
| References: |
*Leeuwenberg J.F.M. et al.: E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77, 543 (1992).
*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
*Nagaraju K. et al.: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin Exp Immunol 113, 407 (1998).
*Tachimoto H. et al., Eotaxin-2 Alters Eosinophil Integrin Function via Mitogen-Activated Protein Kinases. Am J Respir Cell Mol Biol 26, 645 (2002).
*Burdick MM et al.: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am J Physiol Cell Physiol 284, C977 (2003).
*Hertel L. et al.: Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J Virol. 77, 7563 (2003).
*Bacáková L, Mares V, Lisá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9.
*Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V: Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture. J Biomed Mater Res. 2000 Mar 5;49(3):369-79.
*Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36.
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