| Clone: | MEM-154 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The antibody MEM-154 reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes. HLDA V; WS Code M MA068 HLDA V; WS Code NK NK51 |
| Immunogen: | Human granulocytes |
| Species Reactivity: | Human |
| Application: |
Flow Cytometry
Recommended dilution: 5-10 μg/ml
Positive control: PBL (peripheral blood lymphocytes) Application note: The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.
Immunoprecipitation
Western Blotting
Application note: Non-reducing conditions.
Functional Application
The antibody MEM-154 blocks binding of human IgG to FcgammaRIII.
|
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype. |
| References: |
*Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20;270(3):1350-61.
*Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159(8):3940-8.
*Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15;170(6):3054-8.
*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
*de Haas M, Koene HR, Kleijer M, de Vries E, Simsek S, van Tol MJ, Roos D, von dem Borne AE: A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of
human IgG by NK cell Fc gamma RIIIa. J Immunol. 1996 Apr 15;156(8):3948-55.
*Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81.
|
For laboratory research only, not for drug, diagnostic or other use. EXBIO Praha | Nad Safinou II 366 | 252 42 Vestec u Prahy | Czech Republic Tel: +420 261 090 664 | Fax: +420 261 090 660 | orders@exbio.cz | www.exbio.cz |