| Clone: | MEM-98 |
|---|---|
| Isotype: | Mouse IgG1 |
| Specificity: | The MEM-98 antibody reacts with CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells. |
| Immunogen: | Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone). |
| Species Reactivity: | Human |
| Application: |
Flow Cytometry
Recommended dilution: 2 μg/ml
Immunoprecipitation
Western Blotting
Application note: Non-reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: spleen |
| Purity: | > 95% (by SDS-PAGE) |
| Purification: | Purified from ascites by protein-A affinity chromatography. |
| Concentration: | 1 mg/ml |
| Storage Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage / Stability: | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Expiration: | See vial label |
| Lot Number: | See vial label |
| Background: | CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells. |
| References: |
*Osorio LM, De Santiago A, Aguilar-Santelises M, Mellstedt H, Jondal M.: CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic
leukemia B cells from apoptosis induced by anti-IgM. Blood. 1997 Apr 15;89(8):2833-41.
*Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG.: Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation
induced by dendritic cells. Blood. 2006 Apr 15;107(8):3212-20. Epub 2005 Dec 13.
*Bazil V. et al: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol. (Praha) 35, 35 (1989).
*Hassan NJ, Simmonds SJ, Clarkson NG, Hanrahan S, Puklavec MJ, Bomb M, Barclay AN, Brown MH: CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76. Mol Cell Biol. 2006 Sep;26(17):6727-38.
*Castro MA, Oliveira MI, Nunes RJ, Fabre S, Barbosa R, Peixoto A, Brown MH, Parnes JR, Bismuth G, Moreira A, Rocha B, Carmo AM: Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse. J Immunol. 2007 Apr 1;178(7):4351-61.
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